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CBG and CBGa Cannabinoids Professor DeBacco Research Article Russo, E. B. (2011). Taming THC: potential cannabis synergy and phytocannabinoid‐terpenoid entourage effects. British journal of pharmacology, 163(7), 1344-1364. https://www.researchgate.net/publication/51485891_Taming_THC_Potential_cannabis_synergy_and_phytocannabinoid-terpenoid_entourage_effects CBGA Pathway CBGA is produced from GPP and OA by the synthase “Aromatic Prenyltransferase” or AP. This biochemistry takes place in the plastid, which is also where the MEP pathway produces GPP and terpenes. GPP is a shared substrate for both cannabinoids and terpenes. After CBGA is synthesized, it is transported out of the plastid in a “hyaline” glob (Mahlberg), into the apoplast (Gulck). This is where the conversion of CBGA to subsequent cannabinoids takes place. If CBGA is not converted into a subsequent cannabinoid, it will accumulate in the trichome. Heat may cause it to convert to its neutral, active form, CBG. Interaction with Cannabinoid Receptors CBGA and CBG have been shown to interact with cannabinoid receptors that bind CBD and THC, modulating their effect. Anecdotal Reports Many anecdotal reports have shown that CBG has similar effects to CBD. Medical studies have shown that CBG is a “potent adrenoceptor agonist,” (Cascio et al 2010) and other studies have shown that CBG has antibiotic properties (Appendino et al 2008). It is worth noting that all of the general side-chain cannabinoids have been shown to exhibit potent antimicrobial properties. Cannabinoid Biosynthesis CBGA is the cannabinoid precursor The biosynthesis pathway leads through (or can be restricted by) CBGA Link to Lecture Slides: https://drive.google.com/file/d/1txUxTJ9Ho6Z3R4jH5jOYUHjQuzwO2GSm/view?usp=sharing *Due to the description character limit the full work cited for "CBG and CBGa Cannabinoids" can be viewed at... https://drive.google.com/file/d/1wi6esQPT0U7bIZdU5zoJqSstbUTmMCwe/view?usp=sharing

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This is a Non-narrative Cannabis Video Note: I make theses gummies for our military Veteran's, these gummies are given FREE to the Vets, Please Visit My Web Site: Veteran's Cannabis Project - Northwest http://vetscpnw.org/ Ingredients: • 6 oz box of Jell-O, ( Strawberry Jell-O ) • 2 quarter ounce packets Knox unflavored gelatin • 2 tbls. Lemon Juice • 1/4 cup corn syrup • 1/3 cup room temperature Strawberry juice (Fresh strawberries, pureed and strained) • 10 tbls. prepared cannabis tincture (1/2 cup) + 2 tbls. • Corn Starch • Cooking spray -------------------------------------------------------------- Equipment: 1-2 silicone candy molds (I use gummie squares) Small to Medium safe bowl Mixing spoon Measuring cups Airtight container Cooling rack ---------------------------------------------------- Process: Instructions: Combine the Jell-O, unflavored gelatin and Lemon Juice in a medium- bowl and stir until fully blended Add the Strawberry juice and corn syrup. Stir gently, in a sweeping motion, until fully combined. Now let the mixture rest for 5 minutes. Pop the bowl in into a double-boiler on the stove and mix for 5 minutes, until hot, DO NOT Boil, until all the solids are dissolved. Pull mixture off stove and allow it to cool for 4-5 minutes Add your tincture. potency gummies. 6-8 mg THC,per gummie Let the bowl rest for another 5 minutes, gently stirring every couple of minutes, It should become translucent as it sits. This recipe makes roughly 126 gummy squares. Spray the molds with cooking spray. Then fill the mold(s) . Fill to top of each square. Let the gummies rest on the counter for a 1/2 hour. Let the gummies rest in refrigerator for a 1/2 hour. Pop them out of the mold. Place on cookie cooling rack, allow to dry out for 1-2 hours. Place them in an airtight container. I place the gummies in the Freezer, they will not completely freeze due to the tincture Please note: The song is the property and copyright of its rightful owner(s) and _we have NO claims to the ownership. This is a non-profit video made solely for the purpose of study, review or critical analysis purposes only. Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for fair use for purposes such as criticism, comment, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational or personal use tips the balance in favour of fair use. video made solely for the purpose of study, review or critical analysis purposes only.

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Wow…having Dean on was a total pleasure and what an amazing discussion. Yes, we talked weed…cannabis, hemp, CBD, THC and the list goes on. Dean manages “High Life Distributors” out of Oregon and seems to have this cannabis thing figured OUT! Thank you for your time and I look forward to you all’s comments! ___________________________________________________________________ The Unlighten Me Experience podcast - Discussing human behavior, science, politics, exploration, conspiracies and so much more. Dean can be found on: Facebook – www.Facebook.com/dean.donlon Instagram – www.Instagram.com/thehighlifedean

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